High dose vitamin C inhibits proliferation of breast cancer cells through reducing glycolysis and protein synthesis / 浙江大学学报·医学版

OBJECTIVE@#To investigate the effects of high dose vitamin C (VC) on proliferation of breast cancer cells and to explore its mechanisms.@*METHODS@#Human breast cancer cells Bcap37 and MDA-MB-453 were treated with VC at low dose (0.01 mmol/L), medium dose (0.10 mmol/L) and high dose (2.00 mmol/L). Cell proliferation was determined with CCK-8 assay, protein expression was evaluated by Western blot, and the secretion of lactic acid in tumor cells was detected by colorimetric method. Bcap37 cells were inoculated in nude mice, and tumor baring nude mice were intraperitoneally injected with high VC(4 g/kg, VC group, =5)or normal saline (control group, =5) for 24 d. Tumor weight and body weight were calculated.@*RESULTS@# experiments demonstrated that high dose VC significantly inhibited cell proliferation in Bcap37 and MDA-MB-453 cells (all <0.01); the expressions of Glut1 and mTOR signaling pathway-related proteins were decreased (all <0.05); and the secretion of lactic acid was also markedly reduced (all <0.05). experiment showed that the tumor weight was decreased in mice treated with high-dose VC as compared with control group (<0.05), but no difference in body weights between two groups was observed.@*CONCLUSIONS@#High dose VC may inhibit proliferation of breast cancer cells both and through reducing glycolysis and protein synthesis.

Akt Inhibitor Perifosine Prevents Epileptogenesis in a Rat Model of Temporal Lobe Epilepsy / 神经科学通报·英文版

Accumulating data have revealed that abnormal activity of the mTOR (mammalian target of rapamycin) pathway plays an important role in epileptogenesis triggered by various factors. We previously reported that pretreatment with perifosine, an inhibitor of Akt (also called protein kinase B), abolishes the rapamycin-induced paradoxical increase of S6 phosphorylation in a rat model induced by kainic acid (KA). Since Akt is an upstream target in the mTOR signaling pathway, we set out to determine whether perifosine has a preventive effect on epileptogenesis. Here, we explored the effect of perifosine on the model of temporal epilepsy induced by KA in rats and found that pretreatment with perifosine had no effect on the severity or duration of the KA-induced status epilepticus. However, perifosine almost completely inhibited the activation of p-Akt and p-S6 both acutely and chronically following the KA-induced status epilepticus. Perifosine pretreatment suppressed the KA-induced neuronal death and mossy fiber sprouting. The frequency of spontaneous seizures was markedly decreased in rats pretreated with perifosine. Accordingly, rats pretreated with perifosine showed mild impairment in cognitive functions. Collectively, this study provides novel evidence in a KA seizure model that perifosine may be a potential drug for use in anti-epileptogenic therapy.

Effects of Honokiol on cognitive function in mice with kainic acid-induced epilepsy / 浙江大学学报·医学版

OBJECTIVE@#To investigate the effects of Honokiol on cognitive function in mice with epilepsy.@*METHODS@#Kainic acid (38 mg/kg) was intraperitoneally injected in 5 weeks old male ICR mice to induce epilepsy. Honokiol at dose of 3, 10, 30 mg/kg was given to epilepic mice by intraperitoneal injection for 10 days. Fluoro-Jade B staining was used to assess neuronal death; Morris water maze and Y maze tests were used to measure cognitive function such as learning and memory; Western blot was performed to detect the expression of acetylated superoxide dismutase (SOD), microtubule associated protein 1 light chain 3-Ⅱ (LC3-Ⅱ) and P62 in hippocampus tissue; thiobarbituric acid and WST-1 methods were used to detect malondialdehyde (MDA) and SOD.@*RESULTS@#Compared with control group, the levels of acetylated-SOD, MDA, LC3-Ⅱ, P62 and neuronal death increased, cognitive function and SOD decreased in model group (<0.05 or <0.01). Honokiol at the dose of 10 mg/kg and 30 mg/kg decreased SOD acetylation, MDA content, expression of LC3-Ⅱ and P62, as well as neuronal death, and the cognitive function was improved (<0.05 or <0.01), especially in 30 mg/kg Honokiol group.@*CONCLUSIONS@#Honokiol alleviates oxidative stress and autophagy degradation disorder, decreases neuronal death, and therefore improves cognitive function in epilepsy mice.

Efficacy of brain-targeted rapamycin for treatment of epilepsy in rats / 浙江大学学报·医学版

OBJECTIVE@#To investigate the efficacy of brain-targeted rapamycin (T-Rap) in treatment of epilepsy in rats.@*METHODS@#Rapamycin nanoparticles targeting brain were prepared. The epilepsy model was induced by injection of pilocarpine in rats. The rats with pilocarpine-induced epilepsy were treated with rapamycin (Rap group) or brain-targeted rapamycin (T-Rap group). Seizure activity was observed by electroencephalography; the effect on mTOR signaling pathway was detected by Western blot; neuronal death and moss fiber sprouting were analyzed by Fluoro-Jade B (FJB) and Timm's staining, respectively.@*RESULTS@#Electroencephalography showed that both preparation of rapamycin significantly reduced the frequency of spontaneous seizures in rats, and the effect of T-Rap was stronger than that of conventional rapamycin (<0.05). Western blot showed that the phosphorylation levels of S6K and S6 in T-Rap group were lower than those in Rap group (all <0.05), indicating that T-Rap had a stronger inhibitory effect on mTOR signaling pathway. FJB staining showed that T-Rap significantly decreased neuronal death, but there was no significant difference as compared with Rap group. Timm's staining showed that both preparations of rapamycin significantly reduced the germination of mossy fibers, while the effect of T-Rap was more pronounced than Rap group (<0.05). The inhibition of body weight gain of T-Rap group was less than that of Rap group (<0.05).@*CONCLUSIONS@#T-Rap has a better therapeutic effect on epilepsy than conventional rapamycin with a less adverse effects in rats.

Advances in the research of application of urine output monitoring in prevention and treatment of burn shock / 中华烧伤杂志

Fluid therapy is a crucial treatment for patients with extensive burn, which affects patients′prognosis directly. Accurate urine output measurement plays an irreplaceable role in guiding fluid resuscitation in clinic. As one of the best indexes in traditional burn resuscitation, urine output comprehensively reflects systemic circulation. However, it doesn′t fully reflect all the specific chapters of microcirculation and systemic circulation and deficient cellular oxygen metabolism exactly. We need to use urine output combined with other shock parameters to ensure adequate fluid replacement. Currently, the most common way of urine output monitoring is manual measurement. The article reviews the application of urine output monitoring in guiding fluid resuscitation of burn shock.

Clinical efficacy of percutaneous transluminal angioplasty in treatment of renal artery stenosis resulting from fibromuscular dysplasia / 中国介入影像与治疗学

Objective To investigate the efficacy and safty of percutaneous transluminal angioplasty (PTA) of renal artery stenosis resulting from fibromuscular dysplasia.Methods A total of 32 patients with fibromuscular dysplasia complicated with hypertension in level 2 to 3 or refractory hypertension were enrolled,who underwent PTA.The blood pressure,types of medication,restenosis,survival rates and complications were observed after treatment.Results Technical success rate of PTA was 93.94％ (31/33).The blood pressure decreased from (189.6±26.0)mmHg/(121.6± 21.7)mmHg before operation to (136.6±8.0)mmHg/(81.1±11.5)mmHg after operation (t＝9.117,7.745,both P＜ 0.001).No renal artery rupture,interlayer,branch blockage,thrombosis and other related complications and postoperative adverse events occurred.All patients were followed up for 5 months to 100 months,with an average of (40.4±26.1) months.Survival rate was 100％ (32/32).Conclusion PTA is safe and effective for treating renal artery stenosis from fibromuscular dysplasia.

Effects of reflection diary training on nurse's clinical decision-making capability / 现代临床护理

Objective To investigate the effects of reflection diary training on nurse's clinical decision-making capability.Methods A total of 489 nurses in our hospital were enrolled in the study for tests of their clinical decision capability between July 2013 and July 2015.Two hundred forty-eight of them were set as the experiment group and two hundred forty-one of them were set as the control group at random.The reflection diary training was applied in the experiment group for one year.Result After intervention,the scores on clinical decision-making ability,clinical thinking,knowledge structure,ability in nurse-patient communication were all significantly higher than those of control group (P＜0.01).Conclusion The reflection diary training can improve nurse's clinical decision-making capability and inspire their clinical thinking about the nursing problems.

Impact of anti-hypertension therapy on seasonal variability of blood pressure, urinary 8-OHdG levels in essential hypertension / 中国综合临床

Objective To investigate anti-hypertension threapy on seasonal variability of blood pressure,urinary 8-OHdG levels in essential hypertension in order to provide a basis for seasonal antihypertensive treatment.Methods Fifty hypertensive patients admitted the hospital of Erdos Clinical Medical College of Inner Mongolia Medical University at summer 2013 were selected as our subjects.The final subjects was 42 cases due to 8 lost cases.The patients were randomly divided into two groups,including 30 cases in renin angiotensin aldosterone system inhibitors(RASI) group and 12 cases in Ca2+ channel blocker(CCB) group.Epidata 3.1 software was applied to perform statistic analysis.Urinary 8-OHdG concentration was measured by enzyme-linked immunosorbent assay (ELISA).Blood pressure was measured in spring,summer,autumn and winter.Results Systolic blood pressure(SBP) in RASI group and CCB group at winter periods were (158±20) mmHg,(158 ± 20) mmHg,higher than that in summer periods ((145 ± 12) mmHg,(141 ± 9) mmHg;P＜ 0.05).Diastolic blood pressure(DPB) in RASI group and CCB group at winter periods were (101 ± 13)mmHg and (100±4)mmHg,significant high than that in summer periods ((93 ±7) mmHg,(90±7) mmHg;P＜0.05).8-OhdG levels in RASI group at summer and autumn periods were (243.20±30.94) ng/L and (240.40±47.99) ng/L,significantly higher than that in winter and spring periods((190.80± 15.56) ng/L and (189.06± 13.56) ng/L),and the differences were significant(P＜0.001).No significant differences were seen in CCB group among 4 seasons in terms of 8-OhdG levels (P ＞ 0.05).Conclusion Blood pressure change still occur among 4 seasons in hypertensive patients after a single CCB containing RASI-based drug antihypertensive therapy.And blood pressure in winter periods is higher than that in summer,which indicates that therapy medication based on RASI might reduce the level of oxidative stress at winter periods.

A processor of multifunctional truck-mounted water for disaster medical rescue / 局解手术学杂志

Objective To develop a equipment of vehicular multifunctional water,and the problem of water in disaster medical rescue was solved. Methods According to the features of the field water in earthquakes,floods and other disasters,a kind of disaster relief in the wild integrated production,supplying softening water and water purification technology was created,and a system by the pretreatment system, ion exchange,reverse osmosis systems,and storage and transportation system and other parts of the small truck-mounted multifunctional water treatment equipment was developed. Results The vehicular prototypes of multifunctional water treatment machine was developed,the pond water and river water were tested to prove that the throughput of the equipment in production and supply in the land of disaster relief,the puri-fied water and softening water reached 2 080 L/day and 12 100 L/day,which could meet the conducted large-scale medical treatment de-mand. Conclusion The successful design of the vehicular multifunctional water processor carried out the medical rescue with water prob-lem,and provided a new method and new equipment,which was worthy of popularization and application.

The Effect of Insulin Secretagogues on Apoptosis of Pancreatic Beta Cells INS-1 / 医学研究杂志

0.05),Glibenclamide(0.1?mol/L,1?mol/L,10?mol/L) increased cell′s apoptosis by2.35,2.71,2.94 folders(P

Detection of Pseudomonas aeruginosa with Piezoelectric Immunosensor Microarray:An Experimental Research / 中华医院感染学杂志

OBJECTIVE To study the main bacteria and their drug-resistance of postoperative infections after liver transplantation.METHODS The distribution and drug-resistance profile of 156 strains of bacterial isolates from(various) specimens in 68 liver transplantation inpatients were retrospectively analyzed last year.RESULTS The(incidence) of infections after liver transplantation was 67.6 %.The major bacteria were Klebsilla pneumoniae((17.3%)),Staphylococcus aureus(14.1%),Candida albicans(11.0%),and Pseudomonas aeruginosa((11.0%)).The bacteria had the character of multidrug-resistance and high drug-resistance.The most effective(antibiotics) to Gram-negatives and Gram-positives were still the carbopenems and glycopeptide.CONCLUSIONS(Infection) is a major complication after liver transplantation.Prevention,early diagnosis and treatment of the(infection) are very important.

Investigation of quality of life of liver transplantation recipients / 第三军医大学学报

Objective To assess the effects of orthotopic liver transplantation (OLT) on the quality of life (QoL) of the patients with end stage liver disease. Methods QoL of OLT recipients was assessed by the GLQI index of the digestive system disease. A total of 43 recipients with benign end stage liver disease without operational complications, who were clearly conscious and had no difficulty in comprehending the investigation table because of their relative good education above the middle school, had been investigated during May 2000 to December 2002. The detecting data were divided into three parts including before OLT, at discharge, and 3 months after discharge. Results At the stage of discharge, QoL of OLT recipients was improved as compared with that before OLT. The physical function, psychological status, symptom of the recipients had been significantly improved than as compared with those before OLT ( P 0.05). And at the stage of 3 months after discharge, QoL of the recipients was improved significantly than that in the hospital ( P

Transfusion of Cryopreserved Platelets to Surgical Patients / 中国输血杂志

Objective To estimate the efficacy and safety of cryopreserved platelets in surgical patients.Methods 238 patients in recent years with hemorrhage or high risk of hemorrhage induced by thrombocytopenia or functional platelet defects during or after surgery, who had been transfused with cryopreserved platelets, were included in our study. The following items were recorded: bleeding time before and two hours after transfusion of cryopreserved platelets; the persist time of blood oozing from wound after transfusion; platelet count before and one hour after transfusion; platelet count before and immediately after surgery; and the number of patients with adverse transfusion reactions. The hemostatic efficacy was judged by the bleeding time and platelet count change.Results Bleeding times before and two hours after cryopreserved platelets transfusion were (9?3)min,(4?2)min respectively; blood oozing from wound stopped 0.5～2 hours after transfusion; the platelet level was elevated by(5～42)?10 9/L one hour after transfusion; the postoperative platelet count was decreased by(130?69)?10 9/L compared to preoperative count; four patients had urticaria and five had febrile reaction. The hemostatic efficacy was 95%(226/238) Conclusion Cryopreserved platelets function immediately to bring about the cessation of blood oozing from the wound,and elevation of peripheral blood platelet count.

EFFECT OF CRYOPRESERVED PLATELETS TRANSFUSION ON 1560 PATIENTS / 解放军医学杂志

In this study, cryopreservated platelets were transfused to 1560 patients. of them, 536 were patients with acute leukaemia, 285 with anemia, 253 with thrombocytopenia due to chemical or radicalized treat, 438 in perioperation, and 48 with DIC or hemorrhage of rupture esophagus varix. The storage time of cryopreserved platelets ranged from one week to thirteen months. Most of the cryopreserved platelets had not been washed before application and no obvious adverse-effects were observed except for garlic smell in patients' exhalation after transfusing cryopreserved platelets. The results showed that transfusion of cryopreserved platelets has a remarkable on effect hemostasis and can enhance the platelet count in peripheral blood. Compared with liquid-preserved platelets, cryopreserved platelets for clinic use have the following advantages:1) the potentiality of large stock, 2) much longer shelf life, 3) higher safety, 4) superior hemostasis effect, and 5) capacity to meet emergent massive clinic demand.